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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 920-1036 mapping at the C-terminus of ACK of human origin
- recommended for detection of ACK of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine
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ACK Background Information
The Ras-related Rho subfamily of GTP-binding proteins (p21s), which includes Rho, Rac and Cdc42Hs, is implicated in different aspects of cytoskeletal organization. These proteins resemble Ras p21 in that their active GTP-bound form is inactivated by intrinsic hydrolysis of the GTP to GDP, which can be stimulated by GTPase-activating proteins (GAPs). ACK, a tyrosine kinase that specifically binds Cdc42Hs in its GTP-bound form, has been described. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain and inhibits both the intrinsic and GAP-stimulated GTPase activity of Cdc42Hs. These findings suggest that ACK may represent a new class of proteins that sustains the GTP-bound active form of the Rho subfamily of GTP binding proteins and which is directly linked to a tyrosine phosphorylation pathway.
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ACK (H-172)
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Western blot analysis of ACK expression in EOC 20 (A,E), MM-142 (B,D) and H4 (C) whole cell lysates. Antibodies tested include ACK (H-172): sc-9165 (A,B) and ACK (A-11): sc-28336 (C,D,E). ACK (H-172): sc-9165. Western blot analysis of ACK expression in non-transfected: sc-117752 (A) and truncated mouse ACK transfected: sc-118200 (B) 293T whole cell lysates. |
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