recommended for detection of Streptavidin by WB; permits the formation of antibody-Streptavidin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with Avidin
Streptavidin Background Information Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin Biotin with one of the strongest known biological and noncovalent interactions. Each monomer of Streptavidin binds one molecule of Biotin. The strong Streptavidin-Biotin bond can be used to “glue” various chemicals onto surfaces and to link together molecules such as radioisotopes and monoclonal antibodies. Streptavidin is widely utilized in scientific laboratories, commonly for the purification of immunochemistries. It is one of the most important components in diagnostics and laboratory kits.
