epitope corresponding to amino acids 778-827 mapping near the C-terminus of MAN1 of human origin
recommended for detection of MAN1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
MAN1 Background Information The nuclear envelope separates the nucleoplasm from the cytoplasm in eukaryotic cells and includes the outer and inner nuclear membrane, nuclear pore complexes and the nuclear lamina. The nuclear lamina contains intermediate filament-type proteins called lamins that form a dense network to strengthen and stabilize the nuclear envelope. MAN1 is a nuclear envelope protein and shares a constant amino-terminal region called the LAP2-emerin-MAN1 (LEM) motif with nuclear envelope proteins LAP2 and emerin. MAN1 belongs to the MAN antigen family identified by autoantibodies from a patient with collagen vascular disease. A nucleoplasmic N-terminal domain of MAN1 is necessary for inner nuclear membrane retention. The gene encoding human MAN1 maps to chromosome 12q14. LAP2 is another nuclear envelope protein with a LEM motif. Alternative splicing produces six isoforms of mammalian LAP2. LAP2a and LAP2b associate with chromosomal barrier-to-autointegration factor (BAF) and may play a role in stabilizing chromatin structure. LAP2b also binds to lamin B. LAP2a is a non-membrane isoform of LAP2 that associates with the internal nucleoskeleton and binds Lamin A.
