epitope mapping within an extracellular domain of ECE-2 of human origin
recommended for detection of ECE-2, isoforms A, B, and C of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
ECE-2 Background Information Endothelin converting enzymes (ECE-1 and ECE-2) are type II metalloproteases that convert big endothelin-1 to vasoactive endothelin-1. Both ECE-1 and ECE-2 belong to the peptidase family M13 and are Type II membrane proteins. There are several different isoforms of each ECE protein and the expression of the protein may therefore vary. All isoforms are expressed in umbilical endothelial cells, atrium cardiomyocites and ventricles, polynuclear neutrophils and fibroblasts. Endothelin-converting enzyme-2 (ECE-2) converts big endothelin-1 to endothelin-1 by catalyzing the cleavage of the precursor into active peptides, including cleavage at trp21-val22. The ECE-2 gene produces unique isoforms that share the same extracellular catalytic domain and contain unique N-terminal cytoplasmic tails.
