epitope mapping within an internal region of CHP of human origin
recommended for detection of CHP of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with CHP2; also reactive with additional species, including equine, canine, bovine, porcine and avian
CHP Background Information CHP (Calcineurin homologous protein), also known as Calcium-binding protein p22 and Calcineurin B homolog, is a 195 amino acid protein that shares significant sequence similarity to calmodulin and PP2B-A∫, a calmodulin-regulated protein phosphatase. Required for constituitive membrane traffic, CHP functions as a monomer and is expressed in fetal eye, muscle, kidney, liver, lung, speen and thymus. Decreased phosphorylation of CHP is assoicated with an increase in exchange activity. Overexpression of CHP inhibits GTPase-stimulated NHE-1 activity, impairs nuclear translocation and transcriptional activity of NFAT and inhibits phosphatase acivity of calcineurin in a dose-dependent manner.
