Mass Spectrometry Internal Standards

4-Benzyloxy-α-cyanocinnamic acid
Matrix substance for MALDI-MS.
Katalog # CAS Nummer Summenformel Menge
sc-238777 162882-36-2 C₁₇H₁₃NO₃ 250 mg
1-Stearoyl-2-Arachidonoyl PC-d8
Contains eight deuterium atoms at the 5, 6, 8, 9, 11, 12, 14, and 15 positions. It is intended for use as an internal standard for the quantification of SAPC by GC- or LC-mass spectrometry. Oxidation of phospholipids in low-density lipoprotein (LDL) generates pro-inflammatory and pro-thrombotic mediators that may play a crucial role in cardiovascular and inflammatory diseases. 1-stearoyl-2-arachidonoyl-sn-Glycero-3-phosphocholine (SAPC) is one of the major phospholipid species in LDL. Upon oxidation, SAPC has been shown to induce monocyte-endothelial interactions.
Katalog # CAS Nummer Summenformel Menge
sc-220502 35418-59-8 (unlabeled) C₄₆H₇₆D₈NO₈P 50 µg/100 µg
1-Stearoyl-2-Arachidonoyl-sn-Glycerol-d8
SAG-d8 contains eight deuterium atoms at the 5, 6, 8, 9, 11, 12, 14, and 15 positions. Intended for use as an internal standard for the quantification of SAG by GC- or LC-mass spectrometry. Many protein kinase C (PKC) isoforms require activation via second messengers including Ca²⁺, diacylglycerol (DAG), and/or a phospholipid in order to phosphorylate target proteins, initiating a variety of important signaling cascades. It is a DAG that contains the ω-6 polyunsaturated fatty acid arachidonic acid in the sn-2 position and stearic acid in the sn-1 position of the glycerol backbone. Can potently activate PKCα, ε, and δ at nM concentrations. Independent of PKC signaling, SAG competitively binds to the Ras activator RasGRP with a Ki value of 4.49 µM in Jurkat T-cells.
Katalog # CAS Nummer Summenformel Menge
sc-220503 65914-84-3 (unlabeled) C₄₁H₆₄D₈O₅ 100 µg/500 µg
11-dehydro Thromboxane B2-d4
Contains 4 deuterium atoms at the 3, 3', 4, and 4' positions and is intended for use as an internal standard for the quantification of 11-dehydro TXB2 by GC- or LC-mass spectrometry. TXB2 is released in substantial quantities from aggregating platelets and metabolized during circulation to 11-dehydro TXB2 and 2,3-dinor TXB2.1 11-dehydro TXB2 is one of the main plasma metabolites of TXB2 and can be used as a marker for in vivo TXA2 synthesis.
Katalog # CAS Nummer Summenformel Menge
sc-220574 n.n. C₂₀H₂₈D₄O₆ 25 µg/50 µg
11β-Prostaglandin F2α-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions. Intended for use as an internal standard for the quantification of 11β-PGF2α by GC- or LC-mass spectrometry. The primary plasma metabolite of PGD2 in vivo. Normal human urinary excretion of 11β-PGF2α is about 54 ng/mmol creatinine, which is increased nearly 3-fold during allergen-induced bronchoconstriction in asthmatics. It is equipotent to PGF2α in inducing human bronchial smooth muscle contractions and inhibition of adipose differentiation. 11β-PGF2α inhibits ADP or thrombin-induced human platelet aggregation at concentrations of 0.14 to 2.8 µM.
Katalog # CAS Nummer Summenformel Menge
sc-220586 n.n. C₂₀H₃₄D₄O₅ 50 µg/100 µg
12-epi Leukotriene B4-d4
Contains four deuterium atoms at the 6, 7, 14, and 15 positions. Intended for use as an internal standard for the quantification of 12-epi LTB4 by GC- or LC-mass spectrometry. 12-epi LTB4 is the “unnatural” 12(S) epimer of LTB4. 12-epi LTB4 does not appear in any measurable amount in the LTB4 isomers produced by non-enzymatic hydrolysis of LTA4.1
Katalog # CAS Nummer Summenformel Menge
sc-220588 n.n. C₂₀H₂₈D₄O₄ 25 µg/50 µg
(±)12(13)-EpOME-d4
Contains four deuterium atoms at the 9, 10, 12, and 13 positions. Intended for use as an internal standard for the quantification of latanoprost by GC- or LC-mass spectrometry (MS). (±)12(13)-EpOME is the 12,13-cis epoxide of linoleic acid, generated by neutrophils during the oxidative burst.1 The toxicity and biosynthesis of (±)12(13)-EpOME have not been well differentiated from (±)12(13)-EpOME, but have been presumed to be essentially the same. (±)9(10)-EpOME is generated by neutrophils during the oxidative burst.1 Has been recovered from the lungs of hyperoxic rats and from humans with acute respiratory distress syndrome.
Katalog # CAS Nummer Summenformel Menge
sc-220591 n.n. C₁₈H₂₈D₄O₃ 25 µg/50 µg
13,14-dihydro Prostaglandin E1-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions. intended for use as an internal standard for the quantification of 13,14-dh PGE1 by GC- or LC-mass spectrometry. An inhibitor of ADP-induced platelet aggregation in human platelet-rich plasma and washed platelets with IC₅₀ values of 31 and 21 nM, respectively. 13,14-dh PGE1 is a slightly more potent inhibitor of ADP-induced human platelet aggregation than PGE1 which has an IC₅₀ of 40 nM. Also, 13,14-dh PGE1 activates adenylate cyclase in NCB-20 hybrid cells with a Kact of 668 nM.
Katalog # CAS Nummer Summenformel Menge
sc-220593 n.n. C₂₀H₃₂D₄O₅ 25 µg/50 µg
13,14-dihydro-15-keto Prostaglandin D2-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions and is intended for use as an internal standard for the quantification of 13,14-dihydro-15-keto PGD2 by GC- or LC-mass spectrometry. A metabolite of PGD2 which is formed through the 15-hydroxy PGDH pathway. Recently identified as a selective agonist for the CRTH2/DP2 receptor. Also inhibits ion flux in a canine colonic mucosa preparation. In humans, 13,14-dihydro-15-keto PGD2 is further metabolized to give 11β-hydroxy compounds which have also undergone β-oxidation of one or both side chains. Virtually no 13,14-dihydro-15-keto PGD2 survives intact in the urine.
Katalog # CAS Nummer Summenformel Menge
sc-220595 n.n. C₂₀H₂₈D₄O₅ 25 µg/50 µg
13,14-dihydro-15-keto Prostaglandin E1-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions. Intended for use as an internal standard for the quantification of 13,14-dh-15-k PGE1 by GC- or LC-mass spectrometry. A metabolite of PGE1 with much reduced biological activity. Steady state plasma concentrations are about 10 pg/ml. A weak inhibitor of ADP-induced platelet aggregation in human PRP and washed platelets with IC₅₀ values of 54 and 200 µM.
Katalog # CAS Nummer Summenformel Menge
sc-220596 n.n. C₂₀H₃₀O₅D₄ 25 µg/50 µg
13,14-dihydro-15-keto Prostaglandin E2-d4
An isotopically labeled metabolite of PGE2 (sc-201225), with deuterium atoms substituted at the 3 and 4 methylene carbons.
Katalog # CAS Nummer Summenformel Menge
sc-220597 363-23-5 (unlabeled) C₂₀H₂₈D₄O₅ 25 µg/50 µg
13,14-dihydro-15-keto Prostaglandin F2α-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions. Intended for use as an internal standard for the quantification of 13,14-dihydro-15-keto PGF2α by GC- or LC-mass spectrometry. 13,14-dihydro-15-keto PGF2α is the first prominent plasma metabolite of PGF2α in the 15-hydroxy PGDH pathway. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α.
Katalog # CAS Nummer Summenformel Menge
sc-220598 n.n. C₂₀H₃₀D₄O₅ 50 µg/100 µg
(±)14(15)-EET-d11
Contains 11 deuterium atoms at the 16, 16', 17, 17', 18, 18' ,19, 19' ,20, 20, and 20 positions. Intended for use as an internal standard for the quantification of (±)14(15)-EET by GC- or LC-MS. (±)14,15-DiHETrE is biosynthesized in rat and rabbit liver microsomes by CYP450.1,2 Dramatic increases in urinary (±)14,15-DiHETrE, a (±)14(15)-EET metabolite, have been documented by GC/MS in pregnancy induced hypertension.3
Katalog # CAS Nummer Summenformel Menge
sc-220613 n.n. C₂₀H₂₁D₁₁O₃ 25 µg/50 µg
15-deoxy-Δ12,14-Prostaglandin J2-d4
Contains four deuterium atoms at the 3, 3', 4, and 4' positions. Intended for use as an internal standard for the quantification of 15-deoxy-Δ12,14-PGJ2 by GC- or LC-mass spectrometry. A metabolite of PGJ2 and formed from PGD2 the elimination of two molecules of water. It binds selectively to PPARγ with an EC₅₀ of 2 µM in a murine chimera system. More potent than PGD2, Δ12-PGJ2, and PGJ2 in stimulating lipogenesis in C3H10T1/2 cells. The EC₅₀ for induction of adipocyte differentiation in cultured fibroblasts is 7 µM.
Katalog # CAS Nummer Summenformel Menge
sc-220618 n.n. C₂₀H₂₄D₄O₃ 25 µg/50 µg
20-HETE-d6
20-HETE-d6 contains six deuterium atoms at positions 16, 16', 17, 17', 18 and 18’. It is intended for use as an internal standard for quantification of 20-HETE by GC- or LC-mass spectrometry. 20-HETE is a cytochrome P450 (CYP450) metabolite postulated to play an autacoid role in renal and cerebral vasculature. In rat cerebral microvessels, 20-HETE is a vasoconstrictor that mediates pressure-induced autoregulatory vasoconstriction. 20-HETE is excreted mainly as a glucuronide conjugate. The concentration of free 20-HETE (20-40 pg/ml in human urine) is 10-fold lower than the corresponding concentration of 20-glucuronide.
Katalog # CAS Nummer Summenformel Menge
sc-220839 n.n. C₂₀H₂₆D₆O₃ 25 µg/50 µg
20-hydroxy N-Arachidonoyl Taurine
Arachidonic acid is metabolized by cytochrome P450 (CYP450) 4A ω-hydroxylase to 20-HETE, which acts as a vasoconstrictor in the kidney and brain. During mass spectrometry lipidomics analysis of rat brain, a series of fatty acyl amides of taurine was discovered, arachidonoyl taurine is a prominent member. 20-hydroxy N-Arachidonoyl taurine is a potential CYP450 metabolite of N-arachidonoyl taurine and can possibly activate members of the transient receptor potential (TRP) family of calcium channels.
Katalog # CAS Nummer Summenformel Menge
sc-220840 n.n. C₂₁H₃₅NO₅S 25 µg/50 µg
4-oxo 2-Nonenal-d3
4-oxo 2-Nonenal-d3 (4-ONE-d3) contains three deuterium atoms at the terminal methyl position. It is used for an internal standard for the quantification of 4-ONE by GC- or LC-mass spectrometry (MS). 4-ONE is a lipid peroxidation product derived from oxidized ω-6 polyunsaturated fatty acids such as arachidonic acid and linoleic acid. It exhibits various biological activities such as cytotoxicity, genotoxicity, growth inhibiting activity, chemotactic activity and has been widely used as a marker of lipid peroxidation. 4-ONE is a recently identified product of lipid peroxidation. It actively modifies histidine and lysine residues on proteins and causes protein cross-linking. 4-ONE also modifies 2'-deoxy guanosine, further implicating lipid peroxidation in mutagenesis and carcinogenesis.
Katalog # CAS Nummer Summenformel Menge
sc-220992 n.n. C₉H₁₁D₃O₂ 50 µg/100 µg
(±)5-iPF2α-VI-d11
5-iPF2α-VI-d11 contains 11 deuterium atoms at 16, 16, 17, 17, 18, 18, 19, 19, 20, 20, and 20 positions. It is intended for use as an internal standard for quantification of 5-iPF2α-VI by GC- or LC-mass spectrometry. Isoprostanes are prostaglandin-like products of free-radical induced lipid peroxidation.
Katalog # CAS Nummer Summenformel Menge
sc-221031 n.n. C₂₀H₂₃D₁₁O₅ 10 µg/25 µg
5-OxoETE-d7
5-OxoETE-d7 contains seven deuterium atoms at 6, 8, 9, 11, 12, 14, and 15 positions. It is used as an internal standard for the quantification of 5-oxoETE by GC- or LC-mass spectrometry. 5-OxoETE is a polyunsaturated keto acid formed by the oxidation of 5-HETE in human neutrophils. It stimulates cytosolic calcium levels in neutrophils with an EC₅₀ value of 2 nM. 5-OxoETE. This selectively stimulates the migration and degranulation of eosinophils and activates the mitogen-activated protein kinase (MAPK) pathway in stimulated neutrophils.
Katalog # CAS Nummer Summenformel Menge
sc-221043 n.n. C₂₀H₂₃D₇O₃ 25 µg/50 µg
(±)5(6)-EET-d11
(±)5(6)-EET-d11 contains 11 deuterium atoms 16, 16', 17, 17', 18, 18' ,19, 19' ,20, 20, and 20 positions. It is used as an internal standard for the quantification of (±)5(6)-EET by GC- or LC-mass spectrometry (MS). (±)5(6)-EET is biosynthesized in rat and rabbit liver microsomes by cCYP450.1 In neuroendocrine cells, such as the anterior pituitary and pancreatic islet, (±)5(6)-EET has been implicated in the mobilization of Ca²⁺ and hormone secretion.
Katalog # CAS Nummer Summenformel Menge
sc-221071 n.n. C₂₀H₂₁D₁₁O₃ 10 µg/25 µg
5(S)-HETE-d8
5(S)-HETE-d8 contains eight deuterium atoms at positons 5, 6, 8, 9, 11, 12, 14, and 15. It is used as an internal standard for the quantification of 5(S)-HETE by GC- or LC-mass spectrometry (MS). 5(S)-HETE is produced by the action of 5-lipoxygenase on arachidonic acid to give 5(S)-HpETE, followed by reduction of hydroperoxide. 5(S)-HETE has proliferative and chemotactic effects on granulocytes. When further metabolized to 5-oxoETE, it is a more potent eosinophil chemoattractant than leukotriene B4.
Katalog # CAS Nummer Summenformel Menge
sc-221072 n.n. C₂₀H₂₄D₈O₃ 25 µg/50 µg
5(S),6(R)-Lipoxin A4-d5
5(S),6(R)-Lipoxin A4-d5 (5(S),6(R)-LXA4-d5) contains five deuterium atoms at the 19, 19’, 20, 20, and 20 positions. It is used as an internal standard for quantification of 5(S),6(R)-LXA4 by GC- or LC-mass spectrometry (MS). 5(S),6(R)-Lipoxin A4 is a trihydroxy fatty acid containing a conjugated tetraene, produced by the metabolism of 15-HETE or 15-HpETE with human leukocytes. 5(S),6(R)-Lipoxin A4 is equipotent to leukotriene B4 (LTB4) in inducing superoxide generation in human neutrophils at 0.1 µM. 5(S),6(R)-Lipoxin A4 is associated with several other biological functions including leukocyte activation, natural killer cell, chemotaxis effects, inhibition, and monocyte migration and adhesion.
Katalog # CAS Nummer Summenformel Menge
sc-221077 n.n. C₂₀H₂₇D₅O₅ 10 µg/25 µg
ent-8-iso-15(S)-Prostaglandin F2α-d9
A deuterated version of a biologically active isoprostane produced by the non-enzymatic free radical catalyzed peroxidation of arachidonic acid (sc-200770), that has demonstrated vasoconstrictive activity.
Katalog # CAS Nummer Summenformel Menge
sc-221590 n.n. C₂₀H₂₅D₉O₅ 10 µg/25 µg
Hexadecanal-d5
Contains five deuterium atoms at the 15, 15’, 16, 16 and 16 positions. It is intended for use as an internal standard for the quantification of hexadecanal by GC- or LC-mass spectrometry.
Katalog # CAS Nummer Summenformel Menge
sc-221719 629-80-1 (unlabeled) C₁₆H₂₇D₅O 500 µg/1 mg
Lyso-PAF C-16-d4
Lyso-PAF C-16-d4 contains four deuterium atoms at the 7, 7', 8, and 8' positions of the hexadecyl moiety. It is intended for use as an internal standard for the quantification of PAF C-16 by GC- or LC-mass spectrometry.
Katalog # CAS Nummer Summenformel Menge
sc-221867 n.n. C₂₄H₄₈D₄NO₆P 100 µg/500 µg
N-Docosanoyl Taurine
Several different arachidonoyl amino acid conjugates, including N-arachidonoyl dopamine and N-arachidonoyl-L-serine, have been isolated and characterized from bovine brain. N-Docosanoyl taurine is one of several novel taurine-conjugated fatty acids discovered during mass spectrometry lipidomic analysis of brain and spinal cord from wild-type and fatty acid amide hydrolase (FAAH) knockout mice. The levels of N-docosanoyl taurine were elevated ~12 fold in FAAH-/- mice compared to wild-type mice, indicating that FAAH utilizes N-docosanoyl taurine as a substrate. However, in vitro experiments with purified FAAH indicate that related N-fatty acyl taurines and ethanolamines of similar chain length are hydrolyzed 2,000-50,000 times more slowly by FAAH compared to oleoyl ethanolamide. N-acyl taurines bearing polyunsaturated acyl chains can activate members of the transient receptor potential (TRP) family of calcium channels, including TRPV1 and TRPV4.
Katalog # CAS Nummer Summenformel Menge
sc-222002 n.n. C₂₄H₄₉NO₄S 1 mg /5 mg